0/15human palmitoyl-proteome article0orthologues in palmitoyl-proteomes1targeted study0orthologue in targeted studies1siteCys3Predicted1cysteines in main isoform
Protein general information
UniProt names and subcellular location Protein identifiers Protein names Subcellular locations Serine/threonine-protein kinase H1
Protein serine kinase H1
Source Identifiers UniProt gene names PSKH1 IPI ID IPI00007810 Refseq protein ID NP_006733.1 Ensembl protein ENSP00000291041 Ensembl gene ENSG00000159792 UniProt AC P11801 UniProt ID KPSH1_HUMAN UniProt secondary AC Q9NY19 Protein length Isoform 1 (main): 424 aa Known functions (from UniProt) May be a SFC-associated serine kinase (splicing factor compartment-associated serine kinase) with a role in intranuclear SR protein (non-snRNP splicing factors containing a serine/arginine-rich domain) trafficking and pre-mRNA processing. ATP + a protein = ADP + a phosphoprotein. Activity depends on Ca(2+) concentration. Homodimer. Localized in the brefeldin A-sensitive Golgi compartment, at centrosomes, in the nucleus with a somewhat speckle-like presence, membrane-associated to the endoplasmic reticulum (ER) and the plasma membrane (PM), and more diffusely in the cytoplasm. Found to concentrate in splicing factor compartments (SFCs) within the nucleus of interphase cells. The acylation-negative form may be only cytoplasmic and nuclear. Acylation seems to allow the sequestering to the intracellular membranes. Myristoylation may mediate targeting to the intracellular non-Golgi membranes and palmitoylation may mediate the targeting to the Golgi membranes. Dual acylation is required to stabilize the interaction with Golgi membranes. Expressed in all tissues and cell lines tested with the highest level of abundance in testis. Autophosphorylated on serine residues. Myristoylated. Required for membrane association. Prerequisite for palmitoylation to occur. Palmitoylated. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 1 protein kinase domain.
Cysteine summary and predictions
Pos in alignment 3 62 140 252 258 383 Topology U U U U U U Disulfide no no no no no no SS prediction C C H E C C SS UniProt NA NA NA NA NA NA Isoform 1
Topology U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear Prediction scores From top to bottom: Cysteine position, CSS-Palm 4.0 score, PalmPred score
Color code for cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
Color code for CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
Color code for PalmPred: High confidence (score > 0.4), Medium confidence (0.1 > score > 0.4), Low confidence (score < 0.1), Not predicted.
UniProt AC UniProt ID Orthology source Organism Gene names Description Isoforms # palmitome articles # targeted studies # of distinct techniques # of known sites Predicted Q91YA2 KPSH1_MOUSE Ensembl, InParanoid Mouse Pskh1 Serine/threonine-protein kinase H1 (EC 22.214.171.124) (Protein serine kinase H1) (PSK-H1) 1 0/10 0 0 0 Yes E2RKP1 E2RKP1_CANLF Ensembl Dog PSKH1 Uncharacterized protein 1 0/0 0 0 0 Yes Q0V7M1 KPSH1_BOVIN Ensembl Cow PSKH1 Serine/threonine-protein kinase H1 (EC 126.96.36.199) (Protein serine kinase H1) (PSK-H1) 1 0/0 0 0 0 Yes Q501V0 KPSH1_DANRE Ensembl Zebrafish pskh1, si:ch211-201b11.4, zgc:113034 Serine/threonine-protein kinase H1 homolog (EC 188.8.131.52) 1 0/0 0 0 0 Yes
Biological process Cellular component Molecular function GO:2490514 Protein phosphorylation GO:2490495 Cellular_component
GO:2490513 Plasma membrane
GO:2490496 Endoplasmic reticulum membrane
GO:2490511 Microtubule organizing center
GO:2490506 Golgi apparatus
GO:2490531 Nuclear speck
GO:2490475 Protein binding
GO:2490477 Atp binding
GO:2490466 Protein serine/threonine kinase activity
Palmitoylation hits and sites
Hit ID Study # Reference Hit list Isoform # Sites (ID, position, characterisation techniques) SPalmH:19195 Study 1 - Isoform 1 SPalmS:869 , Cys3 , Point mutation of substrate, Labeling of substrate with [3H]palmitate
Study # Reference Organism Cell type Subcellular location Technique(s) Palmitome study? Study 1 Brede G et al., 2003 Chlorocebus aethiops COS1 Total cell lysate Point mutation of substrate, Labeling of substrate with [3H]palmitate No