Palmitoylation summaryValidated by 2 independant techniques or by a targeted study.
0/1schizosaccharomyces pombe palmitoyl-proteome article5orthologues in palmitoyl-proteomes1targeted study2orthologue in targeted studies1siteCys196PATs : ERFB_SCHPOPredicted3cysteines in main isoform
Protein general information
UniProt names and subcellular location Protein identifiers Protein names Subcellular locations GTP-binding protein rho2 Source Identifiers UniProt gene names rho2, SPAC16.01 Refseq protein ID NP_594569.1 UniProt AC Q10133 UniProt ID RHO2_SCHPO Protein length Isoform 1 (main): 200 aa Known functions (from UniProt) Interacts with pck2. Belongs to the small GTPase superfamily. Rho family. Found at the growing tips of interphase cells and at the septum prior to cytokinesis. Involved in cell morphogenesis, the maintenance of growth direction, control of polarity and of cell wall integrity. Regulates the synthesis of alpha-D-glucan through activation of pck2.
Cysteine summary and predictions
Pos in alignment 19 49 110 162 196 197 Topology U U U U U U Disulfide no no no no no no SS prediction C E C E E E SS UniProt NA NA NA NA NA NA Isoform 1
Topology U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear Prediction scores From top to bottom: Cysteine position, CSS-Palm 4.0 score, PalmPred score
Color code for cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
Color code for CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
Color code for PalmPred: High confidence (score > 0.4), Medium confidence (0.1 > score > 0.4), Low confidence (score < 0.1), Not predicted.
UniProt AC UniProt ID Orthology source Organism Gene names Description Isoforms # palmitome articles # targeted studies # of distinct techniques # of known sites Predicted P06781 RHO2_YEAST OMA Yeast RHO2, YNL090W, N2237 GTP-binding protein RHO2 1 1/1 1 1 0 Yes Q22038 RHO1_CAEEL MetaPhors rho-1, rhoa, Y51H4A.3 Ras-like GTP-binding protein rhoA 1 0/0 0 0 0 No P61585 RHOA_BOVIN MetaPhors Cow RHOA, ARHA, RHO12 Transforming protein RhoA (Gb) (p21) 1 0/0 0 0 0 Yes Q1RMJ6 RHOC_BOVIN MetaPhors Cow RHOC, ARHC Rho-related GTP-binding protein RhoC 1 0/0 0 0 0 Yes P61586 RHOA_HUMAN MetaPhors Human RHOA, ARH12, ARHA, RHO12 Transforming protein RhoA (Rho cDNA clone 12) (h12) 1 4/15 0 4 0 Yes P62745 RHOB_HUMAN MetaPhors Human RHOB, ARH6, ARHB Rho-related GTP-binding protein RhoB (Rho cDNA clone 6) (h6) 1 4/15 2 4 2 Yes P48148 RHO1_DROME MetaPhors Fruit fly Rho1, CG8416 Ras-like GTP-binding protein Rho1 1 0/0 0 0 0 Yes Q6NUX8 RHOAA_DANRE MetaPhors Zebrafish rhoaa Rho-related GTP-binding protein RhoA-A 1 0/0 0 0 0 Yes Q6DHM9 RHOAB_DANRE MetaPhors Zebrafish rhoab, zgc:153713 Rho-related GTP-binding protein RhoA-B 1 0/0 0 0 0 Yes Q6DHE8 RHOAD_DANRE MetaPhors Zebrafish rhoad Rho-related GTP-binding protein RhoA-D 1 0/0 0 0 0 No Q7T2E8 RHOAC_DANRE MetaPhors Zebrafish rhoac Rho-related GTP-binding protein RhoA-C 1 0/0 0 0 0 No Q5XIM3 Q5XIM3_RAT MetaPhors Rat RSA-14-44, rCG_55304 Protein RSA-14-44 (RSA-14-44 protein) 1 0/1 0 0 0 Yes P62747 RHOB_RAT MetaPhors Rat Rhob, Arhb Rho-related GTP-binding protein RhoB 1 0/1 0 0 0 Yes P61589 RHOA_RAT MetaPhors Rat Rhoa, Arha, Arha2 Transforming protein RhoA 1 0/1 0 0 0 Yes B2RYP0 B2RYP0_RAT MetaPhors Rat Rhoc Protein Rhoc (Ras homolog gene family, member C) 1 0/1 0 0 0 Yes P62746 RHOB_MOUSE MetaPhors Mouse Rhob, Arhb Rho-related GTP-binding protein RhoB 1 1/8 0 1 0 Yes Q9QUI0 RHOA_MOUSE MetaPhors Mouse Rhoa, Arha, Arha2 Transforming protein RhoA 1 2/8 0 1 0 Yes Q9CR99 Q9CR99_MOUSE MetaPhors Mouse 4930544G11Rik, mCG_125315 Protein 4930544G11Rik (RIKEN cDNA 4930544G11) 1 0/8 0 0 0 Yes Q62159 RHOC_MOUSE MetaPhors Mouse Rhoc, Arhc Rho-related GTP-binding protein RhoC (Silica-induced gene 61 protein) (SIG-61) 1 0/8 0 0 0 Yes
Biological process Cellular component Molecular function GO:4042495 Establishment or maintenance of cell polarity regulating cell shape
GO:4217124 Small gtpase mediated signal transduction
GO:4042483 Establishment or maintenance of actin cytoskeleton polarity
GO:4042463 Regulation of alpha-glucan biosynthetic process
GO:4184542 Gtp catabolic process
GO:4217122 Plasma membrane
GO:4173139 Cell division site
GO:4042459 Site of polarized growth
GO:4042454 Cell tip
GO:4042460 Protein binding
GO:4217104 Gtp binding
GO:4235856 Gtpase activity
Palmitoylation hits and sites
Hit ID Study # Reference Hit list Isoform # Sites (ID, position, characterisation techniques) SPalmH:11483 Study 1 - - SPalmS:628 , Cys196 , Point mutation of substrate
Study # Reference Organism Cell type Subcellular location Technique(s) Palmitome study? Study 1 Sánchez-Mir L et al., 2014 Schizosaccharomyces pombe Whole organism Non-membrane lysate ABE (Acyl-Biotin Exchange), Cellular localization of substrate by fusion with fluorescent protein, Cleavage of thioester bonds with NH2OH, Inhibition of palmitoylation with 2-bromopalmitate, Over-expression of palmitoyltransferase, Point mutation of substrate No