Palmitoylation summaryValidated by 2 independant techniques or by a targeted study.
High confidence hit in 1 palmitoyl-proteome studies.
1/1yeast palmitoyl-proteome article0orthologues in palmitoyl-proteomes2targeted studies0orthologues in targeted studies0sitePATs : SWF1_YEASTPredicted1cysteines in main isoform
Protein general information
UniProt names and subcellular location Protein identifiers Protein names Subcellular locations T-SNARE affecting a late Golgi compartment protein 1
Source Identifiers UniProt gene names TLG1, YDR468C, D8035.11 Refseq protein ID NP_010756.3 UniProt AC Q03322 UniProt ID TLG1_YEAST UniProt secondary AC D6VT92 Protein length Isoform 1 (main): 224 aa Known functions (from UniProt) SNARE protein (of Qc type) involved in membrane fusion probably in retrograde traffic of cytosolic double-membrane vesicles derived from both, early and possibly late endosomes/PVC (prevacuolar compartment) back to the trans-Golgi network (TGN or late Golgi). It has been reported to function both as a (target membrane) t-SNARE and as a (vesicle) v-SNARE. Upon vesicle tethering to the target membrane, which requires additional proteins, a SNARE-pin is formed. This is a very stable 4 parallel alpha-helical coil bundle consisting of 4 SNARE domains (usually one of each type: Qa, Qb, Qc, and R), of which at least one is anchored in the opposite membrane. The formation of the SNARE-pin is believed to bring the two membranes in close proximity and to provide the energy to drive membrane fusion. Through its interaction with the VFT (or GARP) complex, it may also contribute to vesicle recognition specificity and tethering. Regulation of SNARE-pin formation also seems to depend on the phosphorylation state of the protein, phosphorylation by TPK1 causing inhibition and dephosphorylation by SIT4 activation. Interacts in a SNARE-pin with the SNAREs TLG2 (Qa), VTI1 (Qb), and SNC1 or SNC2 (R). Interacts with VPS51 of the VFT (or GARP) complex. Probably shuttling between TGN and early/late endosome. Phosphorylated at Thr-31 by TPK1 and dephosphorylated by SIT4. Palmitoylated by SWF1, which prevents its recognition and ubiquitination by TUL1 and its subsequent degradation. Belongs to the syntaxin family. Contains 1 t-SNARE coiled-coil homology domain.
Cysteine summary and predictions
Pos in alignment 205 206 Topology T T Disulfide no no SS prediction E H SS UniProt NA NA Isoform 1
Topology U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear Prediction scores From top to bottom: Cysteine position, CSS-Palm 4.0 score, PalmPred score
Color code for cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
Color code for CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
Color code for PalmPred: High confidence (score > 0.4), Medium confidence (0.1 > score > 0.4), Low confidence (score < 0.1), Not predicted.
UniProt AC UniProt ID Orthology source Organism Gene names Description Isoforms # palmitome articles # targeted studies # of distinct techniques # of known sites Predicted Q946Y7 SYP61_ARATH OMA Arabidopsis thaliana SYP61, OSM1, At1g28490, F3M18.7 Syntaxin-61 (AtSYP61) (Osmotic stress-sensitive mutant 1) 1 0/1 0 0 0 No Q9HGN3 TLG1_SCHPO OMA Schizosaccharomyces pombe tlg1, SPBC36B7.07 t-SNARE affecting a late Golgi compartment protein 1 (Syntaxin tlg1) 1 0/1 0 0 0 No
Biological process Cellular component Molecular function GO:5469817 Transport
GO:5469811 Protein transport
GO:5469842 Vesicle-mediated transport
GO:5469838 Golgi vesicle transport
GO:5469843 Intracellular protein transport
GO:5469823 Vesicle fusion
GO:5469820 Snare complex
GO:5469807 Integral to membrane
GO:5469789 Trans-golgi network
GO:5469818 Golgi apparatus
GO:5469798 Early endosome membrane
GO:5469806 Late endosome membrane
GO:5469844 Snap receptor activity
Palmitoylation hits and sites
Hit ID Study # Reference Hit list Isoform # Sites (ID, position, characterisation techniques) SPalmH:15918 Study 1 HC - None SPalmH:7425 Study 2 - - None SPalmH:10427 Study 3 - - None
Study # Reference Organism Cell type Subcellular location Technique(s) Palmitome study? Study 1 Roth AF et al., 2006 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) Isogenic LRB759 Total membrane ABE (Acyl-Biotin Exchange) Yes Study 2 Valdez-Taubas J et al., 2005 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) BY4742 Total cell lysate ABE (Acyl-Biotin Exchange), Cellular localization of substrate by fusion with fluorescent protein, Cleavage of thioester bonds with NH2OH, Knock-out of palmitoyltransferase No Study 3 Roth AF et al., 2006 To be annotated To be annotated To be annotated To be annotated No