Palmitoylation summaryValidated by 2 independant techniques or by a targeted study.
0/1yeast palmitoyl-proteome article0orthologues in palmitoyl-proteomes1targeted study0orthologue in targeted studies0sitePATs : PFA4_YEASTNot predicted
Protein general information
UniProt names and subcellular location Protein identifiers Protein names Subcellular locations Telomere length regulator protein RIF1
RAP1-interacting factor 1
Source Identifiers UniProt gene names RIF1, YBR275C, YBR1743 Refseq protein ID NP_009834.4 UniProt AC P29539 UniProt ID RIF1_YEAST UniProt secondary AC D6VQS1, P89507 Protein length Isoform 1 (main): 1916 aa Known functions (from UniProt) Negatively regulates telomere length by preventing telomere elongation or promoting degradation of the telomere ends. Recruited to telomeres by interaction with the C-terminus of RAP1, which binds directly to telomeric repeat DNA. This may create a negative feedback loop in which the addition of new telomere repeats creates binding sites for inhibitors of telomere length extension. May also influence the balance of transcriptional silencing at telomeres and the silent mating type locus HMR, which is mediated by SIR (Silent Information Regulator) proteins including SIR3 and SIR4. RIF1 competes with SIR proteins for binding to the C-terminus of RAP1. In the absence of RIF1, a limiting cellular pool of SIR proteins may preferentially associate with RAP1 at sub-telomeric loci, causing enhanced telomeric silencing and attenuated silencing of the HMR locus. Interacts with RIF2 and RAP1. Localizes to telomeres. Telomere association begins in G1/S, attains a peak during late G2/S phase of the cell cycle, and is lost during telophase. Localization to telomeres may be increased by telomere uncapping caused by expression of a mutant telomerase RNA subunit (TLC1).
Cysteine summary and predictions
Pos in alignment 71 275 453 466 473 556 578 580 906 982 1022 1089 1102 1292 Topology U U U U U U U U U U U U U U Disulfide no no no no no no no no no no no no no no SS prediction NA NA NA NA NA NA NA NA NA NA NA NA NA NA SS UniProt NA NA NA NA NA NA NA NA NA NA NA NA NA NA Isoform 1
Pos in alignment 1399 1498 1523 1595 1697 Topology U U U U U Disulfide no no no no no SS prediction NA NA NA NA NA SS UniProt NA NA NA NA NA Isoform 1
Topology U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear Prediction scores From top to bottom: Cysteine position, CSS-Palm 4.0 score, PalmPred score
Color code for cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
Color code for CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
Color code for PalmPred: High confidence (score > 0.4), Medium confidence (0.1 > score > 0.4), Low confidence (score < 0.1), Not predicted.
Biological process Cellular component Molecular function GO:5395646 Cell cycle
GO:5395610 Telomere maintenance
GO:6545278 Dna replication initiation
GO:5395648 Chromatin silencing at silent mating-type cassette
GO:5395647 Telomere capping
GO:6545279 Dna double-strand break processing
GO:5395599 Chromosome, telomeric region
GO:5395607 Nuclear chromosome, telomeric region
GO:5395638 Telomeric dna binding
Palmitoylation hits and sites
Hit ID Study # Reference Hit list Isoform # Sites (ID, position, characterisation techniques) SPalmH:11542 Study 1 - Isoform 1 None SPalmH:11563 Study 1 - - None
Study # Reference Organism Cell type Subcellular location Technique(s) Palmitome study? Study 1 Park S et al., 2011 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) To be annotated To be annotated Cellular localization of substrate by fusion with fluorescent protein, Cleavage of thioester bonds with NH2OH, ABE (Acyl-Biotin Exchange) No