Palmitoylation summaryValidated by 2 independant techniques or by a targeted study.
High confidence hit in 1 palmitoyl-proteome studies.
1/1yeast palmitoyl-proteome article0orthologues in palmitoyl-proteomes6targeted studies0orthologues in targeted studies2sitesCys318, Cys319PATs : ERFB_YEASTPredicted1cysteines in main isoform
Protein general information
UniProt names and subcellular location Protein identifiers Protein names Subcellular locations Ras-like protein 2 Source Identifiers UniProt gene names RAS2, ASC1, CTN5, GLC5, YNL098C, N2198 Refseq protein ID NP_014301.1 UniProt AC P01120 UniProt ID RAS2_YEAST UniProt secondary AC D6W181, Q45U01 Protein length Isoform 1 (main): 322 aa Known functions (from UniProt) The S.cerevisiae Ras proteins modulate the activity of the adenylate cyclase catalytic subunit and therefore affect the biosynthesis of cyclic-AMP. Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by guanine nucleotide-exchange factor (GEF) CDC25 and inactivated by GTPase-activating proteins (GAPs) IRA1 and IRA2. Farnesylated by RAM1-RAM2, which is required for targeting RAS2 to the cytoplasmic site of the endoplasmic reticulum, where proteolytic processing of the C-terminus by RCE1 and methylation of the resulting carboxyl group by STE14 occurs. Palmitoylated by the ERF2-SHR5 complex, which is required for proper plasma membrane localization of RAS2. RAS2 is necessary for a normal response to nutrient limitations, in general, disruption of the RAS2 locus results in an overall premature starvation response. Present with 19800 molecules/cell in log phase SD medium. Belongs to the small GTPase superfamily. Ras family.
Cysteine summary and predictions
Pos in alignment 318 319 Topology U U Disulfide no no SS prediction E E SS UniProt NA NA Isoform 1
Topology U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear Prediction scores From top to bottom: Cysteine position, CSS-Palm 4.0 score, PalmPred score
Color code for cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
Color code for CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
Color code for PalmPred: High confidence (score > 0.4), Medium confidence (0.1 > score > 0.4), Low confidence (score < 0.1), Not predicted.
Biological process Cellular component Molecular function GO:5389553 Pseudohyphal growth
GO:5389439 Signal transduction
GO:5389440 Replicative cell aging
GO:5389443 Regulation of protein localization
GO:5389380 Ascospore formation
GO:5389445 Small gtpase mediated signal transduction
GO:5389546 Protein localization to bud neck
GO:5389331 Positive regulation of adenylate cyclase activity
GO:6545059 Positive regulation of pseudohyphal growth
GO:5389511 Activation of adenylate cyclase activity
GO:5389447 Gtp catabolic process
GO:5389402 Positive regulation of transcription by galactose
GO:5389326 Plasma membrane
GO:5389512 Endoplasmic reticulum membrane
GO:5389545 Nucleotide binding
GO:5389526 Gtp binding
GO:5389431 Gtpase activity
Palmitoylation hits and sites
Hit ID Study # Reference Hit list Isoform # Sites (ID, position, characterisation techniques) SPalmH:19301 Study 3 - - None SPalmH:19319 Study 7 - - SPalmS:994 , Cys318 , Point mutation of substrate, Labeling of substrate with [3H]palmitate SPalmS:995 , Cys319 , Point mutation of substrate, Labeling of substrate with [3H]palmitate SPalmH:11541 Study 4 - Isoform 1 SPalmS:685 , Cys318 , Point mutation of substrate SPalmH:19176 Study 6 - - SPalmS:853 , Cys319 , Point mutation of substrate, Labeling of substrate with [3H]palmitate SPalmH:11619 Study 5 - Isoform 1 None SPalmH:15899 Study 1 HC - None SPalmH:10423 Study 2 - - None
Study # Reference Organism Cell type Subcellular location Technique(s) Palmitome study? Study 1 Roth AF et al., 2006 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) Isogenic LRB759 Total membrane ABE (Acyl-Biotin Exchange) Yes Study 2 Roth AF et al., 2006 To be annotated To be annotated To be annotated To be annotated No Study 3 Bartels DJ et al., 1999 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) Whole organism Total cell lysate Cellular localization of palmitoyltransferase by immunofluorescence, Knock-out of palmitoyltransferase, Labeling of substrate with [3H]palmitate No Study 4 Dong X et al., 2003 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) RJY690 Total cell lysate Point mutation of substrate, Cellular localization of substrate by fusion with fluorescent protein, Truncation of substrate, Labeling of substrate with [3H]palmitate No Study 5 Jung V et al., 1995 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) Whole organism Total cell lysate Labeling of substrate with [3H]palmitate No Study 6 Deschenes RJ et al., 1987 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) BJ2169 Total cell lysate Point mutation of substrate, Labeling of substrate with [3H]palmitate No Study 7 Mitchell DA et al., 1994 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) Whole organism Total cell lysate Point mutation of substrate, High-Performance Liquid Chromatography (HPLC), Labeling of substrate with [3H]palmitate No