Palmitoylation summaryValidated by 2 independant techniques or by a targeted study.
High confidence hit in 1 palmitoyl-proteome studies.
1/1yeast palmitoyl-proteome article0orthologues in palmitoyl-proteomes2targeted studies0orthologues in targeted studies2sitesCys546, Cys545PATs : AKR1_YEASTPredicted2cysteines in main isoform
Protein general information
UniProt names and subcellular location Protein identifiers Protein names Subcellular locations Casein kinase I homolog 2
Source Identifiers UniProt gene names YCK2, CKI1, YNL154C, N1755 Refseq protein ID NP_014245.1 UniProt AC P23292 UniProt ID KC12_YEAST UniProt secondary AC D6W129 Protein length Isoform 1 (main): 546 aa Known functions (from UniProt) Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. ATP + a protein = ADP + a phosphoprotein. Palmitoylated by AKR1, which is required for proper plasma membrane localization of YCK2. Present with 6160 molecules/cell in log phase SD medium. Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily. Contains 1 protein kinase domain.
Cysteine summary and predictions
Pos in alignment 163 545 546 Topology U U U Disulfide no no no SS prediction C C C SS UniProt NA NA NA Isoform 1
Topology U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear Prediction scores From top to bottom: Cysteine position, CSS-Palm 4.0 score, PalmPred score
Color code for cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
Color code for CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
Color code for PalmPred: High confidence (score > 0.4), Medium confidence (0.1 > score > 0.4), Low confidence (score < 0.1), Not predicted.
Biological process Cellular component Molecular function GO:5497385 Cell morphogenesis
GO:5497365 Protein phosphorylation
GO:5497355 Response to glucose stimulus
GO:5497428 Plasma membrane
GO:5497381 Cellular bud neck
GO:5497399 Mating projection
GO:5497433 Transferase activity
GO:5497401 Nucleotide binding
GO:5497434 Transferase activity, transferring phosphorus-containing groups
GO:5497431 Kinase activity
GO:5497415 Atp binding
GO:5497435 Protein kinase activity
GO:5497408 Protein serine/threonine kinase activity
Palmitoylation hits and sites
Hit ID Study # Reference Hit list Isoform # Sites (ID, position, characterisation techniques) SPalmH:5939 Study 2 - - SPalmS:67 , Cys546(uncertain) , Point mutation of substrate, Labeling of substrate with [3H]palmitate SPalmS:5 , Cys545(uncertain) , Point mutation of substrate, Labeling of substrate with [3H]palmitate SPalmH:5940 Study 3 - - SPalmS:6 , Cys545 SPalmS:68 , Cys546 SPalmH:15903 Study 1 HC - None
Study # Reference Organism Cell type Subcellular location Technique(s) Palmitome study? Study 1 Roth AF et al., 2006 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) Isogenic LRB759 Total membrane ABE (Acyl-Biotin Exchange) Yes Study 2 Roth AF et al., 2002 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) Isogenic LRB759 To be annotated Cleavage of thioester bonds with β-ME, Point mutation of palmitoyltransferase, Point mutation of substrate, Labeling of substrate with [3H]palmitoyl-CoA No Study 3 Babu P et al., 2004 To be annotated To be annotated To be annotated To be annotated No