Palmitoylation summaryValidated by 2 independant techniques or by a targeted study.
High confidence hit in 1 palmitoyl-proteome studies.
1/1yeast palmitoyl-proteome article9orthologues in palmitoyl-proteomes1targeted study7orthologue in targeted studies0sitePATs : ERFB_YEASTPredicted1cysteines in main isoform
Protein general information
UniProt names and subcellular location Protein identifiers Protein names Subcellular locations Guanine nucleotide-binding protein alpha-2 subunit
Source Identifiers UniProt gene names GPA2, SSP101, YER020W Refseq protein ID NP_010937.3 UniProt AC P10823 UniProt ID GPA2_YEAST UniProt secondary AC D3DLR9 Protein length Isoform 1 (main): 449 aa Known functions (from UniProt) Alpha subunit of the heterotrimeric guanine nucleotide-binding protein (G protein) involved in glucose-induced cAMP signaling. Binds to its cognate transmembrane receptor GPR1, which senses extracellular carbon sources, and activates cAMP-PKA signaling and governs diploid pseudohyphal differentiation and haploid invasive growth. The G protein beta-mimic proteins GPB1 and GPB2 inhibit GPA2-GPR1 coupling, probably to reduce signaling in the absence of glucose. Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by the G protein coupled receptor (GPCR) GPR1, which serves as a guanine nucleotide-exchange factor (GEF), and inactivated by RGS2, acting as a GTPase-activating protein (GAP) for GPA2. G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. GPA2 interacts with the kelch repeat beta-mimic proteins GPB1 and GPB2 and with the gamma subunit GPG1. Interacts with the G protein coupled receptor GPR1. Interacts also with regulators of G protein signaling (RGS) protein RGS2. Myristoylation at Gly-2 and palmitoylation at Cys-4 are required for membrane localization and function of the protein. Present with 4570 molecules/cell in log phase SD medium. Belongs to the G-alpha family. G(q) subfamily.
Cysteine summary and predictions
Pos in alignment 4 189 310 320 Topology U U U U Disulfide no no no no SS prediction E H C E SS UniProt NA NA NA NA Isoform 1
Topology U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear Prediction scores From top to bottom: Cysteine position, CSS-Palm 4.0 score, PalmPred score
Color code for cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
Color code for CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
Color code for PalmPred: High confidence (score > 0.4), Medium confidence (0.1 > score > 0.4), Low confidence (score < 0.1), Not predicted.
UniProt AC UniProt ID Orthology source Organism Gene names Description Isoforms # palmitome articles # targeted studies # of distinct techniques # of known sites Predicted P09471 GNAO_HUMAN OMA Human GNAO1 Guanine nucleotide-binding protein G(o) subunit alpha 2 6/15 3 4 0 Yes P63096 GNAI1_HUMAN OMA Human GNAI1, Numa1 Guanine nucleotide-binding protein G(i) subunit alpha-1 (Adenylate cyclase-inhibiting G alpha protein) 2 4/15 7 2 0 Yes Q9DC51 GNAI3_MOUSE OMA Mouse Gnai3 Guanine nucleotide-binding protein G(k) subunit alpha (G(i) alpha-3) 1 6/8 1 3 0 Yes P04899 GNAI2_HUMAN OMA Human GNAI2, GNAI2B Guanine nucleotide-binding protein G(i) subunit alpha-2 6 11/15 6 7 0 Yes B2RSH2 GNAI1_MOUSE OMA Mouse Gnai1, Gnai-1 Guanine nucleotide-binding protein G(i) subunit alpha-1 (Adenylate cyclase-inhibiting G alpha protein) 1 3/8 1 2 0 Yes P11488 GNAT1_HUMAN OMA Human GNAT1, GNATR Guanine nucleotide-binding protein G(t) subunit alpha-1 (Transducin alpha-1 chain) 1 1/15 0 1 0 No A8MTJ3 GNAT3_HUMAN OMA Human GNAT3 Guanine nucleotide-binding protein G(t) subunit alpha-3 (Gustducin alpha-3 chain) 1 2/15 0 2 0 No P08752 GNAI2_MOUSE OMA Mouse Gnai2, Gnai-2 Guanine nucleotide-binding protein G(i) subunit alpha-2 (Adenylate cyclase-inhibiting G alpha protein) 1 7/8 1 3 0 Yes P19086 GNAZ_HUMAN OMA Human GNAZ Guanine nucleotide-binding protein G(z) subunit alpha (G(x) alpha chain) (Gz-alpha) 1 2/15 2 2 0 Yes Q5T697 Q5T697_HUMAN OMA Human GNAT2, hCG_40256 Guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 2 (Guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 2, isoform CRA_a) 1 0/15 0 0 0 No Q542R8 Q542R8_MOUSE OMA Mouse Gnaz, mCG_5989 Guanine nucleotide binding protein, alpha z subunit, isoform CRA_a (Guanine nucleotide-binding protein G(z) subunit alpha) 1 0/8 0 0 0 Yes A2AE33 A2AE33_MOUSE OMA Mouse Gnat2 Guanine nucleotide binding protein, alpha transducing 2 (Guanine nucleotide-binding protein G(t) subunit alpha-2) 1 0/8 0 0 0 No B2RVZ3 B2RVZ3_MOUSE OMA Mouse Gnat3, mCG_21847 Guanine nucleotide binding protein, alpha transducing 3 (Guanine nucleotide-binding protein G(t) subunit alpha-3) (MCG21847) 1 0/8 0 0 0 No P20612 GNAT1_MOUSE OMA Mouse Gnat1, Gnat-1 Guanine nucleotide-binding protein G(t) subunit alpha-1 (Transducin alpha-1 chain) 1 0/8 0 0 0 No P38408 GNA14_BOVIN OMA Cow GNA14 Guanine nucleotide-binding protein subunit alpha-14 (G alpha-14) (G-protein subunit alpha-14) (G-protein subunit GL1 alpha) 1 0/0 0 0 0 Yes
Biological process Cellular component Molecular function GO:6540844 Metabolic process
GO:5276793 Pseudohyphal growth
GO:5276754 Signal transduction
GO:5276799 Regulation of cell size
GO:5276805 Replicative cell aging
GO:5276767 Ascospore formation
GO:5276855 G-protein coupled receptor signaling pathway
GO:5276820 Hexose mediated signaling
GO:5276857 Gtp catabolic process
GO:5276845 Glucose mediated signaling pathway
GO:5276815 Adenylate cyclase-activating g-protein coupled receptor signaling pathway
GO:5276750 Plasma membrane
GO:5276842 Heterotrimeric g-protein complex
GO:5276835 Extrinsic to internal side of plasma membrane
GO:5276735 Signal transducer activity
GO:5276730 Nucleotide binding
GO:5276765 Metal ion binding
GO:5276724 G-protein beta/gamma-subunit complex binding
GO:5276840 G-protein coupled receptor binding
GO:5276856 Guanyl nucleotide binding
GO:5276737 Gtp binding
GO:5276808 Gtpase activity
Palmitoylation hits and sites
Hit ID Study # Reference Hit list Isoform # Sites (ID, position, characterisation techniques) SPalmH:15913 Study 1 HC - None SPalmH:10425 Study 2 - - None
Study # Reference Organism Cell type Subcellular location Technique(s) Palmitome study? Study 1 Roth AF et al., 2006 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) Isogenic LRB759 Total membrane ABE (Acyl-Biotin Exchange) Yes Study 2 Roth AF et al., 2006 To be annotated To be annotated To be annotated To be annotated No