Palmitoylation summaryValidated by 2 independant techniques or by a targeted study.
0/15human palmitoyl-proteome article0orthologues in palmitoyl-proteomes2targeted studies0orthologues in targeted studies2sitesCys94, Cys98PATs : ZDHC3_MOUSEPredicted1cysteines in main isoform
Protein general information
UniProt names and subcellular location Protein identifiers Protein names Subcellular locations ELMO domain-containing protein 2 Source Identifiers UniProt gene names ELMOD2 IPI ID IPI00217918, IPI00170680 Refseq protein ID NP_714913.1, XP_005262943.1, XP_005262944.1, XP_011530121.1 Ensembl protein ENSP00000326342 Ensembl gene ENSG00000179387 UniProt AC Q8IZ81 UniProt ID ELMD2_HUMAN UniProt secondary AC B2R712, D3DNZ0 Protein length Isoform 1 (main): 293 aa Known functions (from UniProt) Alveolar cells (morphologically type II cells) and alveolar macrophages (at protein level). Expressed in brain, colon, heart, kidney, liver, lung, muscle, placenta, small intestine, spleen, stomach and testis. In lung it is expressed in alveolar macrophages and alveolar walls. Contains 1 ELMO domain. Acts as a GTPase-activating protein (GAP) toward guanine nucleotide exchange factors like ARL2, ARL3, ARF1 and ARF6, but not for GTPases outside the Arf family. Regulates IFN-related antiviral responses.
Cysteine summary and predictions
Pos in alignment 29 94 98 246 285 Topology U U U U U Disulfide no no no no no SS prediction C H H H C SS UniProt NA NA NA NA NA Isoform 1
Topology U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear Prediction scores From top to bottom: Cysteine position, CSS-Palm 4.0 score, PalmPred score
Color code for cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
Color code for CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
Color code for PalmPred: High confidence (score > 0.4), Medium confidence (0.1 > score > 0.4), Low confidence (score < 0.1), Not predicted.
Feature type Isoform 1 ubiquitination 55, 61, 150
UniProt AC UniProt ID Orthology source Organism Gene names Description Isoforms # palmitome articles # targeted studies # of distinct techniques # of known sites Predicted Q8BGF6 ELMD2_MOUSE OrthoDB, OMA, Ensembl, InParanoid Mouse Elmod2 ELMO domain-containing protein 2 1 0/10 0 0 0 No Q08DZ3 ELMD2_BOVIN OrthoDB, OMA, Ensembl Cow ELMOD2 ELMO domain-containing protein 2 1 0/0 0 0 0 No
Biological process Cellular component Molecular function GO:2143039 Phagocytosis
GO:2925005 Defense response to virus
GO:2924997 Regulation of defense response to virus
GO:2924971 Positive regulation of gtpase activity
GO:2924965 Gtpase activator activity
Palmitoylation hits and sites
Hit ID Study # Reference Hit list Isoform # Sites (ID, position, characterisation techniques) SPalmH:19239 Study 1 - Isoform 1 None SPalmH:19237 Study 2 - Isoform 1 SPalmS:908 , Cys94(uncertain) , Point mutation of substrate, Labeling of substrate with [3H]palmitate SPalmS:909 , Cys98(uncertain) , Point mutation of substrate, Labeling of substrate with [3H]palmitate
Study # Reference Organism Cell type Subcellular location Technique(s) Palmitome study? Study 1 Suzuki M et al., 2015 Homo sapiens Huh-7 Total cell lysate Cellular localization of substrate by fusion with fluorescent protein, Cleavage of thioester bonds with NH2OH, ABE (Acyl-Biotin Exchange) No Study 2 Suzuki M et al., 2015 Homo sapiens HEK293T Total cell lysate Point mutation of substrate, Over-expression of palmitoyltransferase, Labeling of substrate with [3H]palmitate No