Palmitoylation summaryValidated by 2 independant techniques or by a targeted study.
1/15human palmitoyl-proteome article1orthologues in palmitoyl-proteomes7targeted studies2orthologues in targeted studies2sitesCys3, Cys5PATs : ZDH17_HUMANPredicted2cysteines in main isoform
Protein general information
UniProt names and subcellular location Protein identifiers Protein names Subcellular locations Disks large homolog 4
Postsynaptic density protein 95
Synapse-associated protein 90
Source Identifiers UniProt gene names DLG4, PSD95 IPI ID IPI00019213, IPI00619928, IPI00790650, IPI00789971, IPI00104093, IPI00072756 Refseq protein ID NP_001356.1, NP_001122299.1, XP_005256549.1 Ensembl protein ENSP00000307471, ENSP00000382425, ENSP00000382428 Ensembl gene ENSG00000132535 UniProt AC P78352 UniProt ID DLG4_HUMAN UniProt secondary AC B7Z1S1, G5E939, Q92941, Q9UKK8 Protein length Isoform 1 (main): 724 aa; Isoform 2 : 767 aa; Isoform 3 : 721 aa Known functions (from UniProt) Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B (By similarity). Interacts through its PDZ domains with ANO2 and NETO1. Interacts through its first two PDZ domains with GRIN2A, GRIN2B, GRIN2C, GRIN2D, ASIC3, certain splice forms of GRIN1, KCND2, CXADR, SYNGAP1, KCNA1, KCNA2, KCNA3, KCNA4, ERBB4, LRRC4; LRRC4B and SEMA4C. Interacts through its first PDZ domain with GRIK2, KCNA4 and CRIPT. Interacts through its second PDZ domain with the PDZ domain of NOS1 or the C-terminus of CAPON. Interacts through its third PDZ domain with NLGN1 and CRIPT, and probably with NLGN2 and NLGN3. Interacts through its guanylate kinase-like domain with DLGAP1/GKAP, DLGAP2, DLGAP3, DLGAP4, MAP1A, BEGAIN, SIPA1L1 and KIF13B. Isoform 2 interacts through an L27 domain with HGS/HRS and the first L27 domain of CASK. Interacts with ADR1B, ANKS1B and PRR7. May interact with HTR2A. Interacts with ADAM22, KLHL17 and LGI1. Interacts with FRMPD4 (via C-terminus). Interacts with LRFN1, LRFN2 and LRFN4, but not with LRFN3 nor LRFN5. Interacts (via N-terminal tandem pair of PDZ domains) with GPER1 (via C-terminus tail motif); the interaction is direct and induces the increase of GPER1 protein levels residing at the plasma membrane surface in a estradiol-independent manner. Interacts (via N-terminus tandem pair of PDZ domains) with NOS1 (via N-terminal domain). Interacts with SHANK3. Interacts with KCNJ4 (By similarity). High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
Cysteine summary and predictions
Pos in alignment 3 5 41 48 442 488 559 605 623 669 684 730 Topology U U U U U U U U U U U U Disulfide no no no no no no SS prediction E E SS UniProt NA NA Isoform 1
Isoform 2 Cys488
Isoform 3 Cys3
Topology U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear Prediction scores From top to bottom: Cysteine position, CSS-Palm 4.0 score, PalmPred score
Color code for cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
Color code for CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
Color code for PalmPred: High confidence (score > 0.4), Medium confidence (0.1 > score > 0.4), Low confidence (score < 0.1), Not predicted.
Feature type Isoform 1 phosphorylation 8, 142, 236, 240, 295, 397, 523, 561, 573, 701
UniProt AC UniProt ID Orthology source Organism Gene names Description Isoforms # palmitome articles # targeted studies # of distinct techniques # of known sites Predicted Q62108 DLG4_MOUSE OMA, Ensembl Mouse Dlg4, Dlgh4, Psd95, Pcdh10 Disks large homolog 4 (Postsynaptic density protein 95) (PSD-95) (Synapse-associated protein 90) (SAP-90) (SAP90) 3 2/9 9 2 2 Yes P31016 DLG4_RAT OMA, Ensembl Rat Dlg4, Dlgh4, Psd95 Disks large homolog 4 (Postsynaptic density protein 95) (PSD-95) (Synapse-associated protein 90) (SAP-90) (SAP90) 1 0/1 8 0 2 Yes F1PYW1 F1PYW1_CANLF Ensembl Dog DLG4 Uncharacterized protein 1 0/0 0 0 0 No F1MM66 F1MM66_BOVIN OMA, Ensembl Cow DLG4 Uncharacterized protein 1 0/0 0 0 0 Yes F1QCS0 F1QCS0_DANRE OMA, Ensembl Zebrafish dlg4 Disks large homolog 4 2 0/0 0 0 0 No
Palmitoylation hits and sites
Hit ID Study # Reference Hit list Isoform # Sites (ID, position, characterisation techniques) SPalmH:20999 Study 5 List - None SPalmH:11648 Study 3 - - SPalmS:764 , Cys3 , Point mutation of substrate SPalmS:765 , Cys5 , Point mutation of substrate SPalmH:17644 Study 4 - Isoform 1 SPalmS:821 , Cys3 , Point mutation of substrate, Labeling of substrate with [3H]palmitate SPalmS:822 , Cys5 , Point mutation of substrate, Labeling of substrate with [3H]palmitate SPalmH:21008 Study 6 - - None SPalmH:21023 Study 7 - - None SPalmH:21079 Study 8 - - None SPalmH:11647 Study 2 - - None SPalmH:7838 Study 1 - - None
Study # Reference Organism Cell type Subcellular location Technique(s) Palmitome study? Study 1 Huang K et al., 2004 To be annotated To be annotated To be annotated Labeling of substrate with [3H]palmitoyl-CoA, Over-expression of palmitoyltransferase No Study 2 Topinka JR et al., 1998 Rattus norvegicus Cerebellar granule cell Total cell lysate Cellular localization of substrate by immunofluorescence, Labeling of substrate with [3H]palmitate No Study 3 Topinka JR et al., 1998 Chlorocebus aethiops COS Total cell lysate Point mutation of substrate, Cleavage of thioester bonds with NH2OH, Labeling of substrate with [3H]palmitate No Study 4 El-Husseini AE et al., 2000 Chlorocebus aethiops COS7 Total cell lysate Point mutation of substrate, Cellular localization of substrate by fusion with fluorescent protein, Labeling of substrate with [3H]palmitate No Study 5 Pinner AL et al., 2016 Homo sapiens Brain Total cell lysate ABE (Acyl-Biotin Exchange) Yes Study 6 El-Husseini Ael-D et al., 2001 Chlorocebus aethiops COS7 Total cell lysate Labeling of substrate with [3H]palmitate No Study 7 Pinner AL et al., 2016 Homo sapiens Brain Total cell lysate Cleavage of thioester bonds with NH2OH, ABE (Acyl-Biotin Exchange) No Study 8 Drisdel RC et al., 2006 To be annotated HEK293 To be annotated Cleavage of thioester bonds with NH2OH, Labeling of substrate with [3H]palmitate, ABE (Acyl-Biotin Exchange) No