Palmitoylation summaryValidated by 2 independant techniques or by a targeted study.
0/1rat palmitoyl-proteome article0orthologues in palmitoyl-proteomes1targeted study0orthologue in targeted studies0sitePATs : ZDHC3_MOUSEPredicted2cysteines in main isoform
Protein general information
UniProt names and subcellular location Protein identifiers Protein names Subcellular locations Protein cornichon homolog 2
Cornichon family AMPA receptor auxiliary protein 2
Source Identifiers UniProt gene names Cnih2 IPI ID IPI00366152, IPI00201960 Refseq protein ID NP_001020303.1 RGD ID 1304930 Ensembl protein ENSRNOP00000027421 Ensembl gene ENSRNOG00000020247 UniProt AC Q5BJU5 UniProt ID CNIH2_RAT Protein length Isoform 1 (main): 160 aa Known functions (from UniProt) Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by regulating their rates of activation, deactivation and desensitization. Blocks CACNG8-mediated resensitization of AMPA receptors. Acts as an auxiliary subunit for AMPA-selective glutamate receptors (AMPARs). Found in a complex with GRIA1, GRIA2, GRIA3, GRIA4, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8 Interacts with CACGN8 (By similarity). Interacts with GRIA1. Also localizes to the cell membrane of extrasynaptic sites (dendritic shafts, spines of pyramidal cells). Brain. Expressed in the neocortex, hippocampal formation, and cerebellum (at protein level). Belongs to the cornichon family.
Cysteine summary and predictions
Pos in alignment 9 17 61 62 79 84 131 137 Topology C T E E T T C C Disulfide no no no no no no no no SS prediction H H H H H H H H SS UniProt NA NA NA NA NA NA NA NA Isoform 1
Topology U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear Prediction scores From top to bottom: Cysteine position, CSS-Palm 4.0 score, PalmPred score
Color code for cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
Color code for CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
Color code for PalmPred: High confidence (score > 0.4), Medium confidence (0.1 > score > 0.4), Low confidence (score < 0.1), Not predicted.
Feature type Isoform 1 phosphorylation 36
UniProt AC UniProt ID Orthology source Organism Gene names Description Isoforms # palmitome articles # targeted studies # of distinct techniques # of known sites Predicted Q22361 CNI1_CAEEL MetaPhors cni-1, cnih-2, T09E8.3 Protein cornichon homolog 1 1 0/0 0 0 0 No O14038 YEY5_SCHPO MetaPhors Schizosaccharomyces pombe SPAC2C4.05 Uncharacterized protein C2C4.05 1 0/1 0 0 0 No Q9P6K6 ERV14_SCHPO MetaPhors Schizosaccharomyces pombe erv14, SPAC30C2.05 ER-derived vesicles protein erv14 1 0/1 0 0 0 Yes Q9C7D7 CNIH1_ARATH MetaPhors Arabidopsis thaliana At3g12180, F28J15.3 Protein cornichon homolog 1 1 0/1 0 0 0 No O35089 CNIH2_MOUSE OrthoDB, Ensembl, InParanoid Mouse Cnih2, Cnil Protein cornichon homolog 2 (CNIH-2) (Cornichon family AMPA receptor auxiliary protein 2) (Cornichon-like protein) 1 0/8 0 0 0 Yes Q8TBE1 CNIH3_HUMAN OrthoDB Human CNIH3 Protein cornichon homolog 3 (CNIH-3) (Cornichon family AMPA receptor auxiliary protein 3) 1 0/15 0 0 0 Yes Q5BL21 CNIH2_DANRE OrthoDB, OMA, Ensembl Zebrafish cnih2, zgc:101874 Protein cornichon homolog 2 (CNIH-2) (Cornichon family AMPA receptor auxiliary protein 2) 1 0/0 0 0 0 Yes P49858 CNI_DROME OrthoDB, Ensembl Fruit fly cni, CG5855 Protein cornichon 1 0/0 0 0 0 No Q6PI25 CNIH2_HUMAN OMA, Ensembl, InParanoid Human CNIH2, CNIL Protein cornichon homolog 2 (CNIH-2) (Cornichon family AMPA receptor auxiliary protein 2) (Cornichon-like protein) 1 0/15 0 0 0 Yes
Biological process Cellular component Molecular function GO:1080726 Localization within membrane
GO:1080706 Intracellular signal transduction
GO:1080729 Regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity
GO:1080695 Synaptic transmission, glutamatergic
GO:1080713 Regulation of membrane potential
GO:1080684 Cell junction
GO:1080669 Endoplasmic reticulum membrane
GO:1080680 Postsynaptic density
GO:1080723 Dendritic shaft
GO:1080721 Dendritic spine
GO:1080724 Postsynaptic membrane
GO:1080689 Alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex
GO:1080665 Protein binding
Palmitoylation hits and sites
Hit ID Study # Reference Hit list Isoform # Sites (ID, position, characterisation techniques) SPalmH:10478 Study 1 - - None
Study # Reference Organism Cell type Subcellular location Technique(s) Palmitome study? Study 1 Oku S et al., 2013 To be annotated To be annotated To be annotated To be annotated No