1/8mouse palmitoyl-proteome article0orthologues in palmitoyl-proteomes0targeted study0orthologue in targeted studies0sitePredicted2cysteines in main isoform
Protein general information
UniProt names and subcellular location Protein identifiers Protein names Subcellular locations Endophilin-A1
SH3 domain protein 2A
SH3 domain-containing GRB2-like protein 2
Source Identifiers UniProt gene names Sh3gl2, Sh3d2a IPI ID IPI00331110, IPI00875665, IPI00649333, IPI00387275, IPI00125920 Refseq protein ID NP_062408.2 MGI ID MGI:700009 Ensembl protein ENSMUSP00000030212 Ensembl gene ENSMUSG00000028488 UniProt AC Q62420 UniProt ID SH3G2_MOUSE UniProt secondary AC Q3UTL7, Q3UY49, Q8BXS6, Q8VBV1 Protein length Isoform 1 (main): 352 aa Known functions (from UniProt) Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature. Monomer; in cytoplasm. Homodimer; when associated with membranes (By similarity). Interacts with SYNJ1 and DNM1. Interacts with MAP4K3; the interaction appears to regulate MAP4K3-mediated JNK activation. Interacts with OPHN1 (By similarity). Interacts with PDCD6IP. Interacts with BIN2 (By similarity). Interacts with ATX2. Interacts with ADAM9 and ADAM15 cytoplasmic tails. Concentrated in presynaptic nerve terminals in neurons. An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes (By similarity). The BAR domain dimer forms a rigid crescent shaped bundle of helices with the pair of second amphipathic helices protruding towards the membrane-binding surface. Belongs to the endophilin family. Contains 1 BAR domain. Contains 1 SH3 domain. Was originally thought to have lysophosphatidic acid acyltransferase activity, but has since been experimentally shown not to have this activity.
Cysteine summary and predictions
Pos in alignment 108 294 295 Topology U U U Disulfide no no no SS prediction C C E SS UniProt NA NA NA Isoform 1
Topology U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear Prediction scores From top to bottom: Cysteine position, CSS-Palm 4.0 score, PalmPred score
Color code for cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
Color code for CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
Color code for PalmPred: High confidence (score > 0.4), Medium confidence (0.1 > score > 0.4), Low confidence (score < 0.1), Not predicted.
Feature type Isoform 1 phosphorylation 262 ubiquitination 76
UniProt AC UniProt ID Orthology source Organism Gene names Description Isoforms # palmitome articles # targeted studies # of distinct techniques # of known sites Predicted Q8AXV1 SH3G2_CHICK Ensembl Chicken SH3GL2, SH3P4 Endophilin-A1 (Endophilin-1) (SH3 domain-containing GRB2-like protein 1) (SH3p4) 1 0/0 0 0 0 Yes Q9US59 SKB5_SCHPO MetaPhors Schizosaccharomyces pombe skb5, SPCC24B10.13 Shk1 kinase-binding protein 5 1 0/1 0 0 0 No Q8VWF1 SH3P2_ARATH MetaPhors Arabidopsis thaliana SH3P2, At4g34660, T4L20.240 SH3 domain-containing protein 2 3 0/1 0 0 0 No Q5W7F2 AGD3_ARATH MetaPhors Arabidopsis thaliana AGD3, SFC, VAN3, At5g13300, T31B5.120, FKD2 ADP-ribosylation factor GTPase-activating protein AGD3 (ARF GAP AGD3) (Protein ARF-GAP DOMAIN 3) (AtAGD3) (Protein FORKED 2) (Protein SCARFACE) (Protein VASCULAR NETWORK 3) 1 0/1 0 0 0 Yes Q8L7W0 SH3P3_ARATH MetaPhors Arabidopsis thaliana SH3P3, At4g18060, F15J5.30 SH3 domain-containing protein 3 1 0/1 0 0 0 No O35179 SH3G2_RAT Ensembl, InParanoid Rat Sh3gl2, Sh3d2a, Sh3p4 Endophilin-A1 (Endophilin-1) (SH3 domain protein 2A) (SH3 domain-containing GRB2-like protein 2) (SH3p4) 1 0/1 0 0 0 Yes Q99962 SH3G2_HUMAN Ensembl Human SH3GL2, CNSA2, SH3D2A Endophilin-A1 (EEN-B1) (Endophilin-1) (SH3 domain protein 2A) (SH3 domain-containing GRB2-like protein 2) 1 0/15 0 0 0 Yes Q8T390 SH3G3_DROME Ensembl, InParanoid Fruit fly endoA, endo, CG14296, EndoA Endophilin-A (SH3 domain-containing GRB2-like protein) 1 0/0 0 0 0 Yes
Biological process Cellular component Molecular function GO:842612 Synaptic vesicle endocytosis
GO:842583 Regulation of receptor internalization
GO:5883726 Extracellular vesicular exosome
GO:842598 Protein binding
GO:842609 Lipid binding
GO:842611 Identical protein binding
Palmitoylation hits and sites
Hit ID Study # Reference Hit list Isoform # Sites (ID, position, characterisation techniques) SPalmH:7757 Study 1 List - None
Study # Reference Organism Cell type Subcellular location Technique(s) Palmitome study? Study 1 Wan J et al., 2013 Mus musculus Brain Total cell lysate ABE (Acyl-Biotin Exchange) Yes