Palmitoylation summaryValidated by 2 independant techniques or by a targeted study.
0/8mouse palmitoyl-proteome article0orthologues in palmitoyl-proteomes3targeted studies0orthologues in targeted studies2sitesCys252, Cys253PATs : ZDHC2_MOUSEPredicted2cysteines in main isoform
Protein general information
UniProt names and subcellular location Protein identifiers Protein names Subcellular locations Regulator of G-protein signaling 7-binding protein
R7 family-binding protein
Source Identifiers UniProt gene names Rgs7bp, D13Bwg1146e, R7bp IPI ID IPI00817008 Refseq protein ID NP_084155.2 MGI ID MGI:106334 Ensembl protein ENSMUSP00000066614 Ensembl gene ENSMUSG00000021719 UniProt AC Q8BQP9 UniProt ID R7BP_MOUSE UniProt secondary AC Q0VF69, Q3UVC4, Q8CBD6, Q9CTP1 Protein length Isoform 1 (main): 257 aa Known functions (from UniProt) Regulator of G protein-coupled receptor (GPCR) signaling. Regulatory subunit of the R7-Gbeta5 complexes that acts by controlling the subcellular location of the R7-Gbeta5 complexes. When palmitoylated, it targets the R7-Gbeta5 complexes to the plasma membrane, leading to inhibit G protein alpha subunits. When it is unpalmitoylated, the R7-Gbeta5 complexes undergo a nuclear/cypolasmic shuttling. May also act by controlling the proteolytic stability of R7 proteins, probably by protecting them from degradation. Interacts with 'R7' family proteins RGS6, RGS7, RGS9 and RGS11. Component of some R7-Gbeta5 complex composed of some R7 protein (RGS6, RGS7, RGS9 or RGS11), Gbeta5 (GNB5) and RGS7BP. Shuttling between the plasma membrane, the cytoplasm and the nucleus is regulated by palmitoylation. Specifically expressed in the central nervous system including the retina but not in other non-neuronal tissues (at protein level). The nuclear localization signal is both required for nuclear localization and palmitoylation. Palmitoylation regulates the cell membrane and nuclear shuttling and the regulation of GPCR signaling. Upon depalmitoylation, it is targeted into the nucleus. Belongs to the RGS7BP/RGS9BP family.
Cysteine summary and predictions
Pos in alignment 53 81 96 122 130 131 144 192 252 253 Topology U U U U U U U U U U Disulfide no no no no no no no no no no SS prediction H C H H H H H E E E SS UniProt NA NA NA NA NA NA NA NA NA NA Isoform 1
Topology U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear Prediction scores From top to bottom: Cysteine position, CSS-Palm 4.0 score, PalmPred score
Color code for cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
Color code for CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
Color code for PalmPred: High confidence (score > 0.4), Medium confidence (0.1 > score > 0.4), Low confidence (score < 0.1), Not predicted.
Feature type Isoform 1 phosphorylation 38, 40, 175 ubiquitination 225
UniProt AC UniProt ID Orthology source Organism Gene names Description Isoforms # palmitome articles # targeted studies # of distinct techniques # of known sites Predicted Q5FVH8 R7BP_RAT OrthoDB, OMA, InParanoid Rat Rgs7bp, R7bp Regulator of G-protein signaling 7-binding protein (R7 family-binding protein) 1 0/1 0 0 0 Yes Q6MZT1 R7BP_HUMAN OrthoDB, OMA, Ensembl, InParanoid Human RGS7BP, R7BP Regulator of G-protein signaling 7-binding protein (R7 family-binding protein) 1 0/15 0 0 0 Yes E2R482 E2R482_CANLF OrthoDB, OMA, Ensembl Dog RGS7BP Uncharacterized protein 1 0/0 0 0 0 Yes Q08DH5 R7BP_BOVIN OrthoDB, OMA, Ensembl Cow RGS7BP, R7BP Regulator of G-protein signaling 7-binding protein (R7 family-binding protein) 1 0/0 0 0 0 Yes Q08BU8 R7BPA_DANRE OrthoDB, OMA, Ensembl Zebrafish rgs7bpa, si:dkey-250l23.3, zgc:154140 Regulator of G-protein signaling 7-binding protein A (R7 family-binding protein A) 1 0/0 0 0 0 Yes F1RB10 F1RB10_DANRE Ensembl Zebrafish rgs7bpb Regulator of G-protein-signaling 7-binding protein B 1 0/0 0 0 0 Yes
Biological process Cellular component Molecular function GO:790597 G-protein coupled receptor signaling pathway
GO:790609 Negative regulation of signal transduction
GO:790595 Plasma membrane
GO:790582 Protein binding
Palmitoylation hits and sites
Hit ID Study # Reference Hit list Isoform # Sites (ID, position, characterisation techniques) SPalmH:10338 Study 1 - - SPalmS:396 , Cys252 SPalmS:397 , Cys253 SPalmH:11582 Study 3 - Isoform 1 SPalmS:714 , Cys252(uncertain) , Point mutation of substrate SPalmS:715 , Cys253(uncertain) , Point mutation of substrate SPalmH:10386 Study 2 - - None
Study # Reference Organism Cell type Subcellular location Technique(s) Palmitome study? Study 1 Drenan RM et al., 2005 Homo sapiens HEK293 Total cell lysate Cellular localization of substrate by fusion with fluorescent protein, Cleavage of thioester bonds with NH2OH, Labeling of substrate with [3H]palmitate, Point mutation of substrate, Thin-layer chromatography No Study 2 Jia L et al., 2011 To be annotated To be annotated To be annotated To be annotated No Study 3 Song JH et al., 2006 Mus musculus NG108-15 Total cell lysate Point mutation of substrate, Cellular localization of substrate by immunofluorescence, Labeling of substrate with [3H]palmitate No