Palmitoylation summaryValidated by 2 independant techniques or by a targeted study.
2/8mouse palmitoyl-proteome articles1orthologues in palmitoyl-proteomes0targeted study0orthologue in targeted studies0siteNot predicted
Protein general information
UniProt names and subcellular location Protein identifiers Protein names Subcellular locations Proliferation-associated protein 2G4
IRES-specific cellular trans-acting factor 45 kDa
Proliferation-associated protein 1
Source Identifiers UniProt gene names Pa2g4, Ebp1, Plfap IPI ID IPI00119305 Refseq protein ID NP_035249.1 MGI ID MGI:894684 Ensembl protein ENSMUSP00000026425 Ensembl gene ENSMUSG00000025364 UniProt AC P50580 UniProt ID PA2G4_MOUSE Protein length Isoform 1 (main): 394 aa; Isoform 2 : 340 aa Known functions (from UniProt) May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly (By similarity). Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site). Together with PTBP1 is required for the translation initiation on the foot-and-mouth disease virus (FMDV) IRES. Regulates cell proliferation, differentiation, and survival. Isoform 1 suppresses apoptosis whereas isoform 2 promotes cell differentiation (By similarity). Isoform 2 interacts with the cytoplasmic domain of non-phosphorylated ERBB3; the interaction requires PKC activity. Interacts with AR. Treatment with HRG leads to dissociation from ERBB3 and increases association with AR. Interacts with nucleolin/NCL. Component of a ribonucleoprotein complex containing at least PA2G4, NCL, TOP1, PABPC2, RPLP0, acetylated histone H1 (HIST1H1A or H1F1), histone H1 2/4, RPL4, RPL8, RPL15, RPL18, RPL18A, RPL21, RPL11, RPL12, RPL28, RPL27, RPLP2 and RPL24. Interacts with HDAC2. Interacts with RB1; the interaction is enhanced upon PA2G4 dephosphorylation (By similarity). Interacts with AKT1 (By similarity). Isoform 1 and isoform 2 interact with RNF20 (By similarity). Isoform 2 interacts with HUWE1. Phosphorylation at Ser-361 by PKC/PRKCD regulates its nucleolar localization.
Cysteine summary and predictions
Pos in alignment 49 85 87 149 179 296 Topology U U U U U U Disulfide no no no no no no SS prediction H H E H C H SS UniProt H NA NA H NA H Isoform 1
Topology U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear Prediction scores From top to bottom: Cysteine position, CSS-Palm 4.0 score, PalmPred score
Color code for cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
Color code for CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
Color code for PalmPred: High confidence (score > 0.4), Medium confidence (0.1 > score > 0.4), Low confidence (score < 0.1), Not predicted.
Feature type Isoform 1 phosphorylation 2, 267, 360, 361 ubiquitination 22, 158, 373, 376
UniProt AC UniProt ID Orthology source Organism Gene names Description Isoforms # palmitome articles # targeted studies # of distinct techniques # of known sites Predicted Q9UQ80 PA2G4_HUMAN OrthoDB, Ensembl, InParanoid Human PA2G4, EBP1 Proliferation-associated protein 2G4 (Cell cycle protein p38-2G4 homolog) (hG4-1) (ErbB3-binding protein 1) 2 3/15 0 4 0 No Q09184 CDB4_SCHPO MetaPhors Schizosaccharomyces pombe cdb4, SPAC23H4.09 Curved DNA-binding protein (42 kDa protein) 1 0/1 0 0 0 No Q6AYD3 PA2G4_RAT OrthoDB, Ensembl, InParanoid Rat Pa2g4, rCG_42472 Proliferation-associated protein 2G4 2 0/1 0 0 0 No E2QW49 E2QW49_CANLF OrthoDB, Ensembl Dog PA2G4 Uncharacterized protein 1 0/0 0 0 0 No Q3ZBH5 Q3ZBH5_BOVIN OrthoDB, Ensembl Cow PA2G4 Proliferation-associated 2G4, 38kDa (Uncharacterized protein) 1 0/0 0 0 0 No Q8AW82 Q8AW82_DANRE OrthoDB, Ensembl Zebrafish pa2g4a, dZ150I12.2-001 Novel protein similar to human proliferation-associated 2G4 protein (PA2G4) (Proliferation-associated 2G4, a) (Uncharacterized protein) 1 0/0 0 0 0 No Q5U0Z2 Q5U0Z2_DROME OrthoDB, InParanoid Fruit fly CG10576 LD30448p 1 0/0 0 0 0 No Q6PHD8 Q6PHD8_DANRE Ensembl Zebrafish pa2g4b Proliferation-associated 2G4 ,b (Uncharacterized protein) 1 0/0 0 0 0 No
Biological process Cellular component Molecular function GO:660874 Cellular process
GO:660820 Transcription, dna-dependent
GO:660848 Regulation of transcription, dna-dependent
GO:660870 Regulation of translation
GO:660865 Rrna processing
GO:660883 Negative regulation of transcription, dna-dependent
GO:660877 Ribonucleoprotein complex
GO:660875 Hydrolase activity
GO:660802 Sequence-specific dna binding transcription factor activity
GO:660795 Dna binding
GO:660804 Rna binding
GO:660879 Ubiquitin protein ligase binding
Palmitoylation hits and sites
Hit ID Study # Reference Hit list Isoform # Sites (ID, position, characterisation techniques) SPalmH:7253 Study 1 List - None SPalmH:12132 Study 2 List - None
Study # Reference Organism Cell type Subcellular location Technique(s) Palmitome study? Study 1 Ren W et al., 2013 Mus musculus 3T3-L1 and epididymal fat pads Total membrane Acyl RAC Yes Study 2 Chesarino NM et al., 2014 Mus musculus MEF Total cell lysate Labeling of substrate with 17-ODYA (17-octadecynoic acid) Yes