Palmitoylation summaryValidated by 2 independant techniques or by a targeted study.
1/10mouse palmitoyl-proteome article0orthologues in palmitoyl-proteomes2targeted studies1orthologues in targeted studies1siteCys3Predicted2cysteines in main isoform
Protein general information
UniProt names and subcellular location Protein identifiers Protein names Subcellular locations Nitric oxide synthase, inducible
Inducible NO synthase
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2
Source Identifiers UniProt gene names Nos2, Inosl IPI ID IPI00125726 Refseq protein ID NP_035057.1, NP_001300850.1, NP_001300851.1 MGI ID MGI:97361 Ensembl protein ENSMUSP00000018610 Ensembl gene ENSMUSG00000020826 UniProt AC P29477 UniProt ID NOS2_MOUSE UniProt secondary AC O70515, O70516, Q5SXT3, Q6P6A0, Q8R410 Protein length Isoform 1 (main): 1144 aa Known functions (from UniProt) Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (By similarity). 2 L-arginine + 3 NADPH + 4 O(2) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H(2)O. Binds 1 FAD. Binds 1 FMN. Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme. Not stimulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity. Homodimer. Binds SLC9A3R1 (By similarity). Interacts with GAPDH. Interacts with S100A8 and S100A9 to form the iNOS-S100A8/9 transnitrosylase complex (By similarity). Macrophages. By treatment with endotoxins or cytokines. By lipopolysaccharides (LPS) (in vitro). Expression in the liver oscillates in a circadian manner with peak levels occurring during the late night. Belongs to the NOS family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain.
Cysteine summary and predictions
Pos in alignment 3 33 104 109 194 211 222 284 361 378 451 565 592 629 Topology U U U U U U U U U U U U U U Disulfide no no no no no no no no no no no no no no SS prediction C C C E C C H H C C C C C H SS UniProt NA NA NA NA NA NA H H NA H NA NA NA NA Isoform 1
Pos in alignment 675 788 796 816 857 938 953 971 1010 1076 Topology U U U U U U U U U U Disulfide no no no no no no no no no no SS prediction H C E C C E E E C E SS UniProt NA NA NA NA NA NA NA NA NA NA Isoform 1
Topology U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear Prediction scores From top to bottom: Cysteine position, CSS-Palm 4.0 score, PalmPred score
Color code for cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
Color code for CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
Color code for PalmPred: High confidence (score > 0.4), Medium confidence (0.1 > score > 0.4), Low confidence (score < 0.1), Not predicted.
Feature type Isoform 1 phosphorylation 708, 733, 903, 1049 mono-methylation 422, 716, 1041
UniProt AC UniProt ID Orthology source Organism Gene names Description Isoforms # palmitome articles # targeted studies # of distinct techniques # of known sites Predicted O19114 NOS2_RABIT MetaPhors Oryctolagus cuniculus NOS2 Nitric oxide synthase, inducible (EC 126.96.36.199) (Inducible NO synthase) (Inducible NOS) (iNOS) (NOS type II) (Peptidyl-cysteine S-nitrosylase NOS2) (Fragment) 1 0/0 0 0 0 Yes Q27995 NOS2_BOVIN MetaPhors Cow NOS2 Nitric oxide synthase, inducible (EC 188.8.131.52) (Inducible NO synthase) (Inducible NOS) (iNOS) (NOS type II) (NOSII) (Peptidyl-cysteine S-nitrosylase NOS2) 1 0/0 0 0 0 Yes Q90703 NOS2_CHICK MetaPhors Chicken NOS2 Nitric oxide synthase, inducible (EC 184.108.40.206) (Inducible NO synthase) (Inducible NOS) (iNOS) (Macrophage NOS) (NOS type II) (Peptidyl-cysteine S-nitrosylase NOS2) 1 0/0 0 0 0 Yes M0RDG2 M0RDG2_RAT OrthoDB, Ensembl Rat Nos2, LOC497963 Protein LOC497963 2 0/2 0 0 0 Yes P35228 NOS2_HUMAN OrthoDB, OMA, Ensembl, InParanoid Human NOS2, NOS2A Nitric oxide synthase, inducible (EC 220.127.116.11) (Hepatocyte NOS) (HEP-NOS) (Inducible NO synthase) (Inducible NOS) (iNOS) (NOS type II) (Peptidyl-cysteine S-nitrosylase NOS2) 2 0/15 1 0 1 Yes E1BKI3 E1BKI3_BOVIN OrthoDB Cow NOS1 Nitric oxide synthase 1 0/0 0 0 0 No J9P395 J9P395_CANLF OrthoDB Dog NOS1 Uncharacterized protein 1 0/0 0 0 0 No A2BIN8 A2BIN8_DANRE OrthoDB, Ensembl Zebrafish nos2a Uncharacterized protein 1 0/0 0 0 0 No Q27571 NOS_DROME OrthoDB, Ensembl Fruit fly Nos, CG6713 Nitric oxide synthase (EC 18.104.22.168) (dNOS) 7 0/0 0 0 0 Yes F1PUQ2 F1PUQ2_CANLF OMA, Ensembl Dog NOS2 Nitric oxide synthase,-inducible 1 0/0 0 0 0 Yes F1MYR5 F1MYR5_BOVIN OMA, Ensembl Cow NOS2 Nitric oxide synthase,-inducible 1 0/0 0 0 0 Yes F1RJ31 F1RJ31_PIG OrthoDB, OMA Pig NOS2 Nitric oxide synthase,-inducible 1 0/0 0 0 0 No F1QVQ6 F1QVQ6_DANRE OrthoDB Zebrafish nos1 Nitric oxide synthase 2 0/0 0 0 0 Yes F1LSH9 F1LSH9_RAT OMA, Ensembl Rat LOC497963 Protein LOC497963 1 0/2 0 0 0 Yes F1QML0 F1QML0_DANRE OMA, Ensembl Zebrafish nos2b Uncharacterized protein 1 0/0 0 0 0 Yes J9NVC2 J9NVC2_CANLF Ensembl Dog NOS2 Nitric oxide synthase,-inducible 1 0/0 0 0 0 Yes
Palmitoylation hits and sites
Hit ID Study # Reference Hit list Isoform # Sites (ID, position, characterisation techniques) SPalmH:19233 Study 3 - Isoform 1 None SPalmH:19234 Study 1 - Isoform 1 SPalmS:903 , Cys3 , Point mutation of substrate, Labeling of substrate with [125I]palmitate SPalmH:11681 Study 2 List - None
Study # Reference Organism Cell type Subcellular location Technique(s) Palmitome study? Study 1 Navarro-Lérida I et al., 2004 Chlorocebus aethiops COS7 Total cell lysate Cellular localization of substrate by fusion with fluorescent protein, Cleavage of thioester bonds with NH2OH, Inhibition of palmitoylation with 2-bromopalmitate, Labeling of substrate with [125I]palmitate, Labeling of substrate with [3H]palmitate, Point mutation of substrate No Study 2 Chesarino NM et al., 2014 Mus musculus DC2.4 Total cell lysate Labeling of substrate with 17-ODYA (17-octadecynoic acid) Yes Study 3 Navarro-Lérida I et al., 2004 Mus musculus C2C12 Total cell lysate Inhibition of palmitoylation with 2-bromopalmitate, Labeling of substrate with [3H]palmitate No