Palmitoylation summaryValidated by 2 independant techniques or by a targeted study.
High confidence hit in 1 palmitoyl-proteome studies.
3/8mouse palmitoyl-proteome articles1orthologues in palmitoyl-proteomes4targeted studies2orthologues in targeted studies2sitesCys3, Cys4PATs : FAS_MOUSEPredicted2cysteines in main isoform
Protein general information
UniProt names and subcellular location Protein identifiers Protein names Subcellular locations Neuromodulin
Axonal membrane protein GAP-43
Calmodulin-binding protein P-57
Growth-associated protein 43
Source Identifiers UniProt gene names Gap43, Basp2 IPI ID IPI00128973 Refseq protein ID NP_032109.1 MGI ID MGI:95639 Ensembl protein ENSMUSP00000099881 Ensembl gene ENSMUSG00000047261 UniProt AC P06837 UniProt ID NEUM_MOUSE Protein length Isoform 1 (main): 227 aa Known functions (from UniProt) This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence (By similarity). Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Cytoplasmic surface of growth cone and synaptic plasma membranes. Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2 (By similarity). Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. Belongs to the neuromodulin family. Contains 1 IQ domain.
Cysteine summary and predictions
Pos in alignment 3 4 Topology U U Disulfide no no SS prediction E E SS UniProt NA NA Isoform 1
Topology U Undefined E Extracellular/Lumenal T Transmembrane C Cytosolic/Nuclear Prediction scores From top to bottom: Cysteine position, CSS-Palm 4.0 score, PalmPred score
Color code for cysteine position: Predicted (high confidence prediction with CSS-Palm or/and PalmPred) / Predicted but not validated (in terms of disulfide bond and cellular location) / Not predicted
Color code for CSS-Palm 4.0: High confidence, Medium confidence, Low confidence, Not predicted.
Color code for PalmPred: High confidence (score > 0.4), Medium confidence (0.1 > score > 0.4), Low confidence (score < 0.1), Not predicted.
Feature type Isoform 1 phosphorylation 36, 41, 86, 88, 89, 95, 96, 103, 110, 122, 128, 133, 138, 139, 142, 144, 145, 172, 180, 189, 192, 193 ubiquitination 19, 26, 30, 37, 56, 67, 69, 80, 81, 104, 113, 114, 125, 136, 199
UniProt AC UniProt ID Orthology source Organism Gene names Description Isoforms # palmitome articles # targeted studies # of distinct techniques # of known sites Predicted P07936 NEUM_RAT OrthoDB, OMA, Ensembl, InParanoid Rat Gap43 Neuromodulin (Axonal membrane protein GAP-43) (Growth-associated protein 43) (Protein F1) 1 0/1 7 0 4 Yes P17677 NEUM_HUMAN OrthoDB, OMA, Ensembl Human GAP43 Neuromodulin (Axonal membrane protein GAP-43) (Growth-associated protein 43) (Neural phosphoprotein B-50) (pp46) 2 1/15 2 1 2 Yes E2R1X9 E2R1X9_CANLF OrthoDB, OMA, Ensembl Dog GAP43 Uncharacterized protein 1 0/0 0 0 0 Yes P06836 NEUM_BOVIN OrthoDB, OMA, Ensembl Cow GAP43 Neuromodulin (Axonal membrane protein GAP-43) (Calmodulin-binding protein P-57) (Growth-associated protein 43) 1 0/0 0 0 0 Yes Q6DG93 Q6DG93_DANRE OrthoDB Zebrafish gap43 Gap43 protein (Uncharacterized protein) 1 0/0 0 0 0 Yes
Biological process Cellular component Molecular function GO:354391 Multicellular organismal development
GO:354423 Regulation of growth
GO:354427 Cell fate commitment
GO:354413 Cell differentiation
GO:354397 Nervous system development
GO:354405 Glial cell differentiation
GO:354409 Axon choice point recognition
GO:354400 Axon guidance
GO:354429 Regulation of filopodium assembly
GO:354412 Cell junction
GO:354389 Plasma membrane
GO:5803222 Postsynaptic density
GO:354426 Cell projection
GO:354435 Cell periphery
GO:5803231 Neuronal postsynaptic density
GO:354418 Filopodium membrane
GO:354380 Calmodulin binding
GO:5803214 Phosphatidylserine binding
GO:5803227 Lysophosphatidic acid binding
GO:5803232 Phosphatidylinositol phosphate binding
Palmitoylation hits and sites
Hit ID Study # Reference Hit list Isoform # Sites (ID, position, characterisation techniques) SPalmH:19299 Study 7 - - None SPalmH:10509 Study 3 - - SPalmS:551 , Cys3(uncertain) SPalmS:552 , Cys4(uncertain) SPalmH:19204 Study 6 - Isoform 1 SPalmS:882 , Cys3 , Point mutation of substrate, Labeling of substrate with [3H]palmitate SPalmS:883 , Cys4 , Point mutation of substrate, Labeling of substrate with [3H]palmitate SPalmH:7581 Study 2 List - None SPalmH:12283 Study 4 List - None SPalmH:17645 Study 5 - - None SPalmH:15621 Study 1 HC - None
Study # Reference Organism Cell type Subcellular location Technique(s) Palmitome study? Study 1 Li Y et al., 2012 Mus musculus Neural stem cell Total cell lysate Labeling of substrate with 17-ODYA (17-octadecynoic acid) Yes Study 2 Wan J et al., 2013 Mus musculus Brain Total cell lysate ABE (Acyl-Biotin Exchange) Yes Study 3 Ueno K, 2000 To be annotated To be annotated To be annotated Point mutation of substrate, Thin-layer chromatography, Labeling of substrate with [3H]palmitate No Study 4 Chesarino NM et al., 2014 Mus musculus MEF Total cell lysate Labeling of substrate with 17-ODYA (17-octadecynoic acid) Yes Study 5 Baker LP et al., 1997 Mus musculus N1E-115 Total cell lysate Cleavage of thioester bonds with DTT, Cleavage of thioester bonds with NH2OH, Labeling of substrate with [3H]palmitate No Study 6 Liu Y et al., 1993 Chlorocebus aethiops COS7 Total cell lysate Point mutation of substrate, Cellular localization of substrate by immunofluorescence, Labeling of substrate with [3H]palmitate No Study 7 Ueno K, 2000 Mus musculus Neuron Total cell lysate Labeling of substrate with [3H]palmitate No